Index of /species/data/dsim/fasta
Name Last modified Size Description
Parent Directory 27-Aug-2008 18:08 -
dsim2chrU_scergenome.megablast.txt 12-Nov-2005 18:45 2.1M
dsim_wu050602.fa.gz 13-Jan-2006 14:04 37.8M
from <URL:ftp://genome.wustl.edu//pub/seqmgr/Drosophila/simulans/simulans_mosaic_assembly/>.
dated 2005Jun02.
README:
This D. simulans assembly represents a mosaic of several different D. simulans lines.
The assembly process began with a ~4X WGS assembly of the the D. simulans w501 line.
The w501 contigs were initially anchored, ordered and oriented by alignment with the
D. melanogaster genome. The assembly was then examined for places where the w501 assembly
suggested inversions with respect to the D. melanogaster assembly. One major inversion was
found, confirming the already documented inversion found by Lemeunier and Ashburner (1976).
Six other D. simulans lines (c167.4, md106ts, md199s, nc48s, sim4, and sim6) were assembled
with ~1X coverage. Using the 4X WGS assembly of the simulans w501 genome as a scaffold,
contigs and unplaced reads from the 1X assemblies of the other individual D. simulans lines
were used to cover gaps in the w501 assembly where possible. Thus the resulting assembly
is a mosaic containing the w501 contigs as the primary scaffolding with contigs and unplaced
reads from the other lines filling gaps in the w501 assembly.
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