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PASA, Program to Assemble Spliced Alignments,
is a eukaryotic genome annotation tool that exploits spliced alignments of expressed transcript sequences to automatically model gene structures, and to maintain gene structure annotation consistent with the most recently available experimental sequence data. PASA also identifies and classifies all splicing variations supported by the transcript alignments.
Statistics & Status reports for Arthropod PASA EST assemblies
Daphnia pulex waterflea
Drosophila melanogaster fruitfly
Nasonia jewel wasp
Acyrthosiphon Pea Aphid
Ixodes tick
Bombyx silkmoth
EST Mapping error distribution
Arthropod EST map errors by Assembly unit size
Data sets for Arthropod PASA EST assemblies
Daphnia ftp://wfleabase.org/daphnia/genome/Daphnia_pulex/current/pasa/
Drosophila ftp://eugenes.org/eugenes/genomes/dmel5/pasa_out/
Nasonia ftp://eugenes.org/eugenes/genomes/nasvit/pasa_out/
Pea Aphid ftp://eugenes.org/eugenes/genomes/aphid/pasa_out/
Ixodes ftp://eugenes.org/eugenes/genomes/ixodes/pasa_out/
Bombyx ftp://eugenes.org/eugenes/genomes/silkworm/pasa_out/
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EST assemblies summary
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| DB Name | aphid | bombyx | daphnia | drosmel | ixodes | nasonia |
| Total EST | 167706 | 245761 | 166289 | 567759 | 194420 | 175853 |
| Any alignment | 157910 | 240085 | 145578 | 561200 | 185783 | 167823 |
| Valid align | 136041 | 209121 | 114128 | 533435 | 130902 | 147382 |
| Assemblies | 24720 | 33194 | 18211 | 42618 | 16206 | 21865 |
| Subclusters | 21066 | 30212 | 15827 | 33329 | 14058 | 17847 |
| Comparison | 2 | 1 | 1 | 1 | 1 | 9 |
| Date | 2009-12-06 | 2009-12-03 | 2008-11-01 | 2008-03-19 | 2009-11-28 | 2009-12-07 |
| Genes w/ EST | 9617 | 8519 | 10595 | 9202 | 7768 | 8676 |
| Incorporated | 5579 | 1267 | 6351 | 7704 | 2659 | 1350 |
| UTR addition | 2577 | 4634 | 2388 | 5423 | 1364 | 5600 |
| Gene extension | 866 | 1096 | 655 | 1062 | 652 | 452 |
| Gene Merging | 344 | 585 | 837 | 1128 | 662 | 760 |
| Gene Splitting | 426 | 0 | 0 | 0 | 0 | 0 |
| Alt splice | 1293 | 1255 | 1102 | 3298 | 888 | 2044 |
| New Gene | 5714 | 18734 | 3094 | 5925 | 6029 | 6000 |
| Antisense | 645 | 178 | 452 | 190 | 230 | 240 |
| Single exon inc. | 1578 | 1621 | 908 | 2049 | 726 | 1317 |
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Percent of assemblies
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| DB Name | aphid | bombyx | daphnia | drosmel | ixodes | nasonia |
| % Incorporated | 23 | 4 | 35 | 18 | 16 | 6 |
| % UTR addition | 10 | 14 | 13 | 13 | 8 | 26 |
| % Gene extension | 4 | 3 | 4 | 2 | 4 | 2 |
| % Gene Merging | 1 | 2 | 5 | 3 | 4 | 3 |
| % Gene Splitting | 2 | 0 | 0 | 0 | 0 | 0 |
| % Alt splice | 5 | 4 | 6 | 8 | 5 | 9 |
| % New Gene | 23 | 56 | 17 | 14 | 37 | 27 |
| % Antisense | 3 | 1 | 2 | 0 | 1 | 1 |
| % Single exon inc. | 6 | 5 | 5 | 5 | 4 | 6 |
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